Use of multispectral data to identify farm intensification levels by applying emergent computing techniques

Concern about feeding an ever increasing population has long been one of humankind’s most pressing problems. This has been addressed throughout history by introducing into farming systems changes allowing them to produce more per unit of land area. However, these changes have also been linked to negative effects on the socio economic and environmental sphere, that have created the need for an integral understanding of this phenomenon. This thesis describes the application of learning machine methods to induct a relationship between the spectral response of farms’ land cover and their intensification levels from a sample of farming of Urdaneta municipality, Aragua state of Venezuela. Data collection like this is a necessary first steep to implement cost-effective methods that can help policymakers to conduct succesful planing tasks, especially in countries such as Venezuela where, in spite of there being areas capable of agricultural production, nearly 50% of the internal food requirements of recent years have been satisfied by importations. In this work, farm intensification levels are investigated through a sample of farms of Urdaneta Municipality, Aragua state of Venezuela. This area is characterised by a wide diversity of farming systems ranging from crop to crop-livestock systems and an increasing population density in regions capable of livestock and arable farming, making it a representative case of the main tropical rural zones. The methodology applied can be divided into two main phases. First an unsupervised classification was performed by applying principal component analysis and agglomerative cluster methods to a set of land use and land management indicators, with the aim to segregate farms into homogeneous groups from the intensification point of view. This procedure resulted in three clusters which were named extensive, semi-intensive and intensive. The land use indicators included the percentage area within each farm devoted to annual crops, orchard and pasture, while the land management indicators were percentage of cultivated land under irrigation, stocking rate, machinery and equipment index and permanent and temporary staff ratio, all of them built from data held on the 1996- 1997 venezuelan agricultural census. The previous clusters reached were compared to the ones obtained by applying the learning machine method known as self-organizing map, which is also an unsupervised classification technique, as a way to confirm the groups’ existence. In the second stage, the learning machine known as kernel adatron algorithm was implemented seeking to identify the intensification level of Urdaneta farms from a landsat image, which consisted of two sequential steps: namely training and validation. In the training step, a predetermined number of instances randomly selected from the data set were analysed looking for a pattern to establish a relationship between the label and the spectral response in an iterative process which was concluded when the machine found a linear function capable of separating the two classes with a maximum margin. The supervised classification finishes with the validation in which the kernel adatron classifies the unseen samples by using a generalisation of the relationships learned while training. Results suggest that farm intensification levels can be effectively derived from multi-spectral data by adopting a machine learning approach like the one described

The recovery of European freshwater biodiversity has come to a halt

Owing to a long history of anthropogenic pressures, freshwater ecosystems are among the most vulnerable to biodiversity loss1. Mitigation measures, including wastewater treatment and hydromorphological restoration, have aimed to improve environmental quality and foster the recovery of freshwater biodiversity2. Here, using 1,816 time series of freshwater invertebrate communities collected across 22 European countries between 1968 and 2020, we quantified temporal trends in taxonomic and functional diversity and their responses to environmental pressures and gradients. We observed overall increases in taxon richness (0.73% per year), functional richness (2.4% per year) and abundance (1.17% per year). However, these increases primarily occurred before the 2010s, and have since plateaued. Freshwater communities downstream of dams, urban areas and cropland were less likely to experience recovery. Communities at sites with faster rates of warming had fewer gains in taxon richness, functional richness and abundance. Although biodiversity gains in the 1990s and 2000s probably reflect the effectiveness of water-quality improvements and restoration projects, the decelerating trajectory in the 2010s suggests that the current measures offer diminishing returns. Given new and persistent pressures on freshwater ecosystems, including emerging pollutants, climate change and the spread of invasive species, we call for additional mitigation to revive the recovery of freshwater biodiversity.publishedVersio

Familial hypercholesterolaemia in children and adolescents from 48 countries: a cross-sectional study

Background: Approximately 450 000 children are born with familial hypercholesterolaemia worldwide every year, yet only 2·1% of adults with familial hypercholesterolaemia were diagnosed before age 18 years via current diagnostic approaches, which are derived from observations in adults. We aimed to characterise children and adolescents with heterozygous familial hypercholesterolaemia (HeFH) and understand current approaches to the identification and management of familial hypercholesterolaemia to inform future public health strategies. Methods: For this cross-sectional study, we assessed children and adolescents younger than 18 years with a clinical or genetic diagnosis of HeFH at the time of entry into the Familial Hypercholesterolaemia Studies Collaboration (FHSC) registry between Oct 1, 2015, and Jan 31, 2021. Data in the registry were collected from 55 regional or national registries in 48 countries. Diagnoses relying on self-reported history of familial hypercholesterolaemia and suspected secondary hypercholesterolaemia were excluded from the registry; people with untreated LDL cholesterol (LDL-C) of at least 13·0 mmol/L were excluded from this study. Data were assessed overall and by WHO region, World Bank country income status, age, diagnostic criteria, and index-case status. The main outcome of this study was to assess current identification and management of children and adolescents with familial hypercholesterolaemia. Findings: Of 63 093 individuals in the FHSC registry, 11 848 (18·8%) were children or adolescents younger than 18 years with HeFH and were included in this study; 5756 (50·2%) of 11 476 included individuals were female and 5720 (49·8%) were male. Sex data were missing for 372 (3·1%) of 11 848 individuals. Median age at registry entry was 9·6 years (IQR 5·8-13·2). 10 099 (89·9%) of 11 235 included individuals had a final genetically confirmed diagnosis of familial hypercholesterolaemia and 1136 (10·1%) had a clinical diagnosis. Genetically confirmed diagnosis data or clinical diagnosis data were missing for 613 (5·2%) of 11 848 individuals. Genetic diagnosis was more common in children and adolescents from high-income countries (9427 [92·4%] of 10 202) than in children and adolescents from non-high-income countries (199 [48·0%] of 415). 3414 (31·6%) of 10 804 children or adolescents were index cases. Familial-hypercholesterolaemia-related physical signs, cardiovascular risk factors, and cardiovascular disease were uncommon, but were more common in non-high-income countries. 7557 (72·4%) of 10 428 included children or adolescents were not taking lipid-lowering medication (LLM) and had a median LDL-C of 5·00 mmol/L (IQR 4·05-6·08). Compared with genetic diagnosis, the use of unadapted clinical criteria intended for use in adults and reliant on more extreme phenotypes could result in 50-75% of children and adolescents with familial hypercholesterolaemia not being identified. Interpretation: Clinical characteristics observed in adults with familial hypercholesterolaemia are uncommon in children and adolescents with familial hypercholesterolaemia, hence detection in this age group relies on measurement of LDL-C and genetic confirmation. Where genetic testing is unavailable, increased availability and use of LDL-C measurements in the first few years of life could help reduce the current gap between prevalence and detection, enabling increased use of combination LLM to reach recommended LDL-C targets early in life

The recovery of European freshwater biodiversity has come to a halt

Owing to a long history of anthropogenic pressures, freshwater ecosystems are among the most vulnerable to biodiversity loss1. Mitigation measures, including wastewater treatment and hydromorphological restoration, have aimed to improve environmental quality and foster the recovery of freshwater biodiversity2. Here, using 1,816 time series of freshwater invertebrate communities collected across 22 European countries between 1968 and 2020, we quantified temporal trends in taxonomic and functional diversity and their responses to environmental pressures and gradients. We observed overall increases in taxon richness (0.73% per year), functional richness (2.4% per year) and abundance (1.17% per year). However, these increases primarily occurred before the 2010s, and have since plateaued. Freshwater communities downstream of dams, urban areas and cropland were less likely to experience recovery. Communities at sites with faster rates of warming had fewer gains in taxon richness, functional richness and abundance. Although biodiversity gains in the 1990s and 2000s probably reflect the effectiveness of water-quality improvements and restoration projects, the decelerating trajectory in the 2010s suggests that the current measures offer diminishing returns. Given new and persistent pressures on freshwater ecosystems, including emerging pollutants, climate change and the spread of invasive species, we call for additional mitigation to revive the recovery of freshwater biodiversity.N. Kaffenberger helped with initial data compilation. Funding for authors and data collection and processing was provided by the EU Horizon 2020 project eLTER PLUS (grant agreement no. 871128); the German Federal Ministry of Education and Research (BMBF; 033W034A); the German Research Foundation (DFG FZT 118, 202548816); Czech Republic project no. P505-20-17305S; the Leibniz Competition (J45/2018, P74/2018); the Spanish Ministerio de Economía, Industria y Competitividad—Agencia Estatal de Investigación and the European Regional Development Fund (MECODISPER project CTM 2017-89295-P); Ramón y Cajal contracts and the project funded by the Spanish Ministry of Science and Innovation (RYC2019-027446-I, RYC2020-029829-I, PID2020-115830GB-100); the Danish Environment Agency; the Norwegian Environment Agency; SOMINCOR—Lundin mining & FCT—Fundação para a Ciência e Tecnologia, Portugal; the Swedish University of Agricultural Sciences; the Swiss National Science Foundation (grant PP00P3_179089); the EU LIFE programme (DIVAQUA project, LIFE18 NAT/ES/000121); the UK Natural Environment Research Council (GLiTRS project NE/V006886/1 and NE/R016429/1 as part of the UK-SCAPE programme); the Autonomous Province of Bolzano (Italy); and the Estonian Research Council (grant no. PRG1266), Estonian National Program ‘Humanitarian and natural science collections’. The Environment Agency of England, the Scottish Environmental Protection Agency and Natural Resources Wales provided publicly available data. We acknowledge the members of the Flanders Environment Agency for providing data. This article is a contribution of the Alliance for Freshwater Life (www.allianceforfreshwaterlife.org).Peer reviewe

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke — the second leading cause of death worldwide — were conducted predominantly in populations of European ancestry1,2. Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis3, and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach4, we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry5. Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries

Gestión e implementación del enfoque de equidad de género e interculturalidad en establecimientos de salud en una zona altoandina, 2022

El presente trabajo tuvo como objetivo principal analizar la gestión e implementación del enfoque de equidad de género e interculturalidad en la atención en establecimientos de salud de una zona altoandina. El enfoque del estudio es cualitativo, interpretativo, tipo básica, nivel exploratorio y diseño etnográfico. La recolección de datos fue mediante la entrevista semiestructurada y observación participante a los profesionales de salud que laboran en establecimientos públicos de zonas altoandinas. Los resultados constataron que las atenciones con ambos enfoques se realizaron principalmente en los servicios de obstetricia y trabajo social, dirigidas en fundamentalmente a las mujeres. Contribuyendo al incremento de la planificación familiar, partos institucionales, disminución de complicaciones de maternas y participación activa de las comunidades. Se concluyó que en los establecimientos de salud de zonas altoandinas los recursos disponibles para la adecuación de infraestructura son limitados, la difusión de las normativas a los gestores y profesionales de la salud es escasa, la frecuencia de capacitación al personal de salud para mejorar los procesos internos y los planes operativos institucionales que comprendan iniciativas con ambos enfoques es baja

”Rethink, Think again and Relearn” : A Qualitative Study of Preschool Teachers´ Perceptions of Läslyftet as a Method for Competence Development in Language and Literacy Didactics.

Mätningar av barn i skolåldern har visat en försämring inom läs- och skrivförmåga. Genom att utveckla och fördjupa lärarnas kompetenser om barns lärande inom språk-, läs- och skrivdidaktik, stärks barnens språkliga förmåga redan från tidig ålder. Denna utveckling och fördjupande av lärarnas kompetenser inom språk-, läs- och skrivdidaktik sker genom Läslyftet. För att synliggöra vad läslyftet har haft för påverkan på förskollärarna och verksamheten är det genom denna studie syftet att beskriva förskollärares uppfattningar om Läslyftet som metod för kompetensutveckling. Frågeställningarna för studien är: Vilka uppfattningar har förskollärare om Läslyftet? På vilket sätt har Läslyftets kollegiala lärande främjat kompetensutveckling inom språk-, läs- och skrivdidaktik? Hur påverkar förskollärares inställning till Läslyftet sin kompetensutveckling inom språk-, läs- och skrivdidaktik?  Ämnet undersöks genom kvalitativa intervjuer där sex förskollärare medverkade. Förskollärarnas uppfattningar om Läslyftet analyserades med hjälp av ramfaktorteorin och Learnings Studies två nivåer om lärande. Utgångspunkterna från ramfaktorteorin är personramen och den fysiska ramen och Learning Studies två nivåer om lärande, lärarens lärande och elevers lärande.  Resultatet visar att det kollegiala lärandet har varit en genomgående faktor för förskollärare att utveckla sina kompetenser inom språk-, läs- och skrivdidaktik. Läslyftets ämnesinnehåll och modell för kompetensutveckling har överskådligt uppfattats som positiv och givande. Förskollärarna uppfattar en förändring i sin inställning kring språk-, läs- och skrivdidaktik samt att det kollegiala har varit meningsfull för en gemensam utveckling för arbetslagen

Use of multispectral data to identify farm intensification levels by applying emergent computing techniques

Concern about feeding an ever increasing population has long been one of humankind’s most pressing problems. This has been addressed throughout history by introducing into farming systems changes allowing them to produce more per unit of land area. However, these changes have also been linked to negative effects on the socio economic and environmental sphere, that have created the need for an integral understanding of this phenomenon. This thesis describes the application of learning machine methods to induct a relationship between the spectral response of farms’ land cover and their intensification levels from a sample of farming of Urdaneta municipality, Aragua state of Venezuela. Data collection like this is a necessary first steep to implement cost-effective methods that can help policymakers to conduct succesful planing tasks, especially in countries such as Venezuela where, in spite of there being areas capable of agricultural production, nearly 50% of the internal food requirements of recent years have been satisfied by importations. In this work, farm intensification levels are investigated through a sample of farms of Urdaneta Municipality, Aragua state of Venezuela. This area is characterised by a wide diversity of farming systems ranging from crop to crop-livestock systems and an increasing population density in regions capable of livestock and arable farming, making it a representative case of the main tropical rural zones. The methodology applied can be divided into two main phases. First an unsupervised classification was performed by applying principal component analysis and agglomerative cluster methods to a set of land use and land management indicators, with the aim to segregate farms into homogeneous groups from the intensification point of view. This procedure resulted in three clusters which were named extensive, semi-intensive and intensive. The land use indicators included the percentage area within each farm devoted to annual crops, orchard and pasture, while the land management indicators were percentage of cultivated land under irrigation, stocking rate, machinery and equipment index and permanent and temporary staff ratio, all of them built from data held on the 1996- 1997 venezuelan agricultural census. The previous clusters reached were compared to the ones obtained by applying the learning machine method known as self-organizing map, which is also an unsupervised classification technique, as a way to confirm the groups’ existence. In the second stage, the learning machine known as kernel adatron algorithm was implemented seeking to identify the intensification level of Urdaneta farms from a landsat image, which consisted of two sequential steps: namely training and validation. In the training step, a predetermined number of instances randomly selected from the data set were analysed looking for a pattern to establish a relationship between the label and the spectral response in an iterative process which was concluded when the machine found a linear function capable of separating the two classes with a maximum margin. The supervised classification finishes with the validation in which the kernel adatron classifies the unseen samples by using a generalisation of the relationships learned while training. Results suggest that farm intensification levels can be effectively derived from multi-spectral data by adopting a machine learning approach like the one described.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Desarrollo de un ensayo casero para la detección de IgG contra el core del virus de la Hepatitis B

Hepatitis B infection affects individuals worldwide, especially in Latin America. Serological assay for HBV antibodies and antigen detection, are critical for HBV diagnostic and treatment. Available commercial ELISA kits are expensive and in our country not always are readily available. The aim of this study was develop a homemade ELISA kit for serological detection IgG anti-HBV core (IgG anti-HBc) accessible for our patients. 114 samples were analyzed: 17 from seronegatives individuals, 48 from seropositives patients and 49 belonging to indigenous population from Mérida State. Exposure to HBV was determined using the Murex anti-HBc test (DiaSorin, UK). Homemade ELISA shows 100% specificity and del 100 % sensitivity, and was in very good agreement with serological status, Kappa= 1 (CI 95%: 0,767-1), X2 (p<0,001). In indigenous population (Wayuu and mestizos), overall IgG anti-HBc prevalence was 22%. Taking into account these results, homemade IgG anti-HBc ELISA is efficacious and low cost usefully, for HBV diagnosis